Sanctuary is a nightclub for vampires and their human 'friends' to socialise, though the latter are typically only present to satisfy the dietary needs of the primary clientele.

In the game, Eric Bane wakes up in Sanctuary and meets Rose, who helps him learn more about what it means to be a vampire, in addition to offering him various missions. The Sanctuary acts as a 'hub' for most of the game, a place to gather intel, grab a drink and pick up a new mission.